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BSI PD ISO/TS 20224-6:2020

BSI PD ISO/TS 20224-6:2020

$142.49

Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR – Horse DNA detection method

Published By Publication Date Number of Pages
BSI 2020 24
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This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of horse-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of horse material derived from domestic horse (Equus caballus), mule (Equus caballus ? × Equus asinus ?), hinny (Equus caballus ? × Equus asinus ?) and zebroid (Equus caballus × Equus simplicidens). The assay also detects the species Przewalski’s horse (Equus przewalskii) and zebra (Equus burchellii).

The target sequence is an Equus caballus isolate Twilight breed thoroughbred chromosome 28, EquCab3.0, whole genome shotgun sequence (i.e. GenBank accession number NC_009171.3)[ 1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ? 95 % replicability at this concentration (LOD95 %).

PDF Catalog

PDF Pages PDF Title
2 undefined
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Scientific basis
5 Reagents and materials
5.1 General
5.2 PCR reagents
11 6 Apparatus
7 Procedure
7.1 Preparation of the test portion/sample
7.2 Preparation of DNA extracts
7.3 PCR setup
7.3.1 Reaction mixes
12 7.3.2 PCR controls
7.3.3 Real-time PCR thermocycler plate set-up
7.4 Temperature-time programme
8 Accept/reject criteria
8.1 General
13 8.2 Identification
9 Validation status and performance criteria
9.1 General
9.2 Robustness
14 9.3 Reproducibility
9.4 Sensitivity
17 9.5 Specificity
19 10 Test report
20 Annex A (informative) BlastN 2.9.0 results for query of GenBank refseq_genomes (452 databases)
23 Bibliography

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BSI PD ISO/TS 20224-6:2020
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