{"id":341060,"date":"2024-10-19T23:54:25","date_gmt":"2024-10-19T23:54:25","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-iso-166542001a12017\/"},"modified":"2024-10-25T23:05:34","modified_gmt":"2024-10-25T23:05:34","slug":"bs-en-iso-166542001a12017","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-iso-166542001a12017\/","title":{"rendered":"BS EN ISO 16654:2001+A1:2017"},"content":{"rendered":"

This International Standard specifies a horizontal method for the detection of Escherichia coli<\/i> serogroup O157.<\/p>\n

Subject to the limitations discussed in the introduction, this International Standard is applicable to products intended for human consumption or for animal feeding stuffs.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
4<\/td>\nEuropean Foreword
Anchor 3
European Foreword to amendment A1 <\/td>\n<\/tr>\n
5<\/td>\nAnnex ZA (normative) Normative references to international publications with their relevant European publications <\/td>\n<\/tr>\n
8<\/td>\nForeword <\/td>\n<\/tr>\n
9<\/td>\nIntroduction <\/td>\n<\/tr>\n
11<\/td>\n1 Scope
2 Normative references
3 Term and definition <\/td>\n<\/tr>\n
12<\/td>\n4 Principle
5 Culture media, reagents and antisera
5.1 Enrichment medium: Modified tryptone soya broth with novobiocin (mTSB + N)
5.1.1 Modified tryptone soya broth (mTSB)
5.1.2 Novobiocin solution <\/td>\n<\/tr>\n
13<\/td>\n5.2 First selective isolation medium: Cefixime tellurite sorbitol MacConkey agar (CT-SMAC)
5.2.1 Base medium
5.2.2 Potassium tellurite solution <\/td>\n<\/tr>\n
14<\/td>\n5.2.3 Cefixime solution
5.2.4 Complete medium
5.3 Second selective isolation medium <\/td>\n<\/tr>\n
15<\/td>\n5.4 Nutrient agar
5.4.1 Composition
5.4.2 Preparation
5.4.3 Preparation of nutrient agar plates
5.5 Tryptone\/tryptophan medium
5.5.1 Composition
5.5.2 Preparation <\/td>\n<\/tr>\n
16<\/td>\n5.6 Kovac\u2019s indole reagent
5.6.1 Composition
5.6.2 Preparation
5.7 Anti-Escherichia coli O157 immunomagnetic particles
5.8 Wash buffer: Modified phosphate buffer, 0,01mol\/l, of pH 7,2
5.8.1 Composition
5.8.2 Preparation
5.9 Normal saline solution
5.9.1 Composition <\/td>\n<\/tr>\n
17<\/td>\n5.9.2 Preparation
6 Apparatus and glassware
6.1 Apparatus for dry sterilization (oven) and\/or wet sterilization (autoclave) <\/td>\n<\/tr>\n
18<\/td>\n6.16 Vortex mixer
7 Sampling
8 Preparation of test sample
9 Procedure (see annex A)
9.1 Test portion and initial suspension
9.2 Enrichment <\/td>\n<\/tr>\n
19<\/td>\n9.3 Immunomagnetic separation (IMS)
9.3.1 General
9.3.2 Immunocapture
9.3.3 Separation
9.4 Plating out onto selective agars and identification of E. coli O157 colonies
9.4.1 Plating out <\/td>\n<\/tr>\n
20<\/td>\n9.4.2 Recognition of typical E. coli O157 colonies
9.5 Confirmation
9.5.1 Selection of colonies
9.5.2 Biochemical confirmation: Indole formation
9.5.3 Serological identification <\/td>\n<\/tr>\n
21<\/td>\n9.6 Further characterization
10 Quality assurance
10.1 Test strains for quality assurance purposes
10.2 Culture method
11 Expression of results
\uea0112 Performance characteristics of the method
12.1 Interlaboratory study
12.2 Sensitivity <\/td>\n<\/tr>\n
22<\/td>\n12.3 Specificity
12.4 LOD50
\uea0113 Test report <\/td>\n<\/tr>\n
23<\/td>\nAnnex A (normative) Diagram of procedure <\/td>\n<\/tr>\n
24<\/td>\n\uea01Annex B (informative) Results of interlaboratory studies <\/td>\n<\/tr>\n
27<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Microbiology of food and animal feeding stuffs. Horizontal method for the detection of Escherichia coli O157<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2017<\/td>\n28<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":341062,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-341060","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/341060","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/341062"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=341060"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=341060"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=341060"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}