This International Standard specifies an in vitro<\/i> procedure to characterize the UVA protection of sunscreen products. Specifications are given to enable determination of the spectral absorbance characteristics of UVA protection in a reproducible manner.<\/p>\n
In order to determine relevant UVA protection parameters, the method has been created to provide a UV spectral absorbance curve from which a number of calculations and evaluations can be undertaken. Results from this measurement procedure can be used for other computations, as required by local regulatory authorities. These include calculation of the Ultraviolet-A protection factor (UVAPF) [correlating with in vivo<\/i> UVAPF from the persistent pigment darkening (PPD) testing procedure], critical wavelength and UVA absorbance proportionality. These computations are optional and relate to local sunscreen product labelling requirements. This method relies on the use of in vivo<\/i> SPF results for scaling the UV absorbance curve.<\/p>\n
This International Standard is not applicable to powder products such as pressed powder and loose powder products.<\/p>\n
| PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
|---|---|---|---|---|---|---|---|
| 6<\/td>\n | Foreword <\/td>\n<\/tr>\n | ||||||
| 7<\/td>\n | 1\tScope 2\tTerms and definitions <\/td>\n<\/tr>\n | ||||||
| 8<\/td>\n | 3\tPrinciple 4\tApparatus 4.1\tUV spectrophotometer specifications <\/td>\n<\/tr>\n | ||||||
| 9<\/td>\n | 4.2\tCalibration of the UV spectrophotometer 4.3\tCalibration of the UV exposure source 4.4\tMonitoring of the UV exposure source <\/td>\n<\/tr>\n | ||||||
| 10<\/td>\n | 4.5\tCalibration of the UVA radiometer used to monitor the test sample irradiation 4.6\tSubstrate\/plate 5\tTest method 5.1\tOutline of the test procedure 5.2\tEquipment calibration and validation of test plates <\/td>\n<\/tr>\n | ||||||
| 11<\/td>\n | 5.3\tAbsorption measurements through the plate 5.4\tSample application 5.5\tAbsorbance measurements of the product-treated plate 5.6\tNumber of determinations 5.7\tDetermination of initial calculated SPF (SPFin vitro), \u201cC\u201d value, initial UVAPF(UVAPF0), and UV exposure dose <\/td>\n<\/tr>\n | ||||||
| 13<\/td>\n | 5.8\tUV exposure 5.9\tMeasurement of final adjusted absorbance spectrum <\/td>\n<\/tr>\n | ||||||
| 14<\/td>\n | 5.10\tCalculation of UVAPF of plates after UV exposure of the sample 6\tProcedure using the spreadsheet in this International Standard <\/td>\n<\/tr>\n | ||||||
| 15<\/td>\n | 7\tReference sunscreen formula S2 8\tTest report <\/td>\n<\/tr>\n | ||||||
| 16<\/td>\n | Annex\u00a0A \n(normative)<\/p>\n Calibration of UV spectrophotometer and plate transmission test <\/td>\n<\/tr>\n | ||||||
| 20<\/td>\n | Annex\u00a0B \n(normative)<\/p>\n Radiometer calibration to spectroradiometric irradiance procedure <\/td>\n<\/tr>\n | ||||||
| 22<\/td>\n | Annex\u00a0C \n(normative)<\/p>\n Computation values: PPD and erythema action spectra and UVA | ||||||
| 25<\/td>\n | Annex\u00a0D \n(normative)<\/p>\n PMMA test plate surface specifications <\/td>\n<\/tr>\n | ||||||
| 27<\/td>\n | Annex\u00a0E \n(normative)<\/p>\n UVA reference sunscreen S2 <\/td>\n<\/tr>\n | ||||||
| 31<\/td>\n | Annex\u00a0F \n(informative)<\/p>\n Statistical calculations <\/td>\n<\/tr>\n | ||||||
| 33<\/td>\n | Bibliography Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Determination of sunscreen UVA photoprotection in vitro<\/b><\/p>\n |