{"id":601858,"date":"2024-11-05T22:12:13","date_gmt":"2024-11-05T22:12:13","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/iso-19040-22018\/"},"modified":"2024-11-05T22:12:13","modified_gmt":"2024-11-05T22:12:13","slug":"iso-19040-22018","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/iso\/iso-19040-22018\/","title":{"rendered":"ISO 19040-2:2018"},"content":{"rendered":"<p>This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with a genetically modified yeast strain Arxula adeninivorans. This reporter gene assay is based on the activation of the human estrogen receptor alpha.<\/p>\n<p>Arxula adeninivorans is a highly robust and salt- and temperature-tolerant test organism and is especially suitable for the analysis of samples with high salinity (conductivity up to 70 mS\/cm). The test organism can be cultivated in medium with sodium chloride content up to 20 %.<\/p>\n<p>This method is applicable to:<\/p>\n<p>\u2014          fresh water;<\/p>\n<p>\u2014          waste water;<\/p>\n<p>\u2014          sea water;<\/p>\n<p>\u2014          brackish water;<\/p>\n<p>\u2014          aqueous extracts and leachates;<\/p>\n<p>\u2014          eluates of sediments (fresh water);<\/p>\n<p>\u2014          pore water;<\/p>\n<p>\u2014          aqueous solutions of single substances or of chemical mixtures;<\/p>\n<p>\u2014          drinking water.<\/p>\n<p>The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 1,5 ng\/l and 3 ng\/l 17\u00df-estradiol equivalents (EEQ). The upper threshold of the dynamic range for this test is between 25 ng\/l and 40 ng\/l 17\u00df-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.<\/p>\n<p>An international interlaboratory trial for the validation of this document has been carried out. The results are summarized in Annex F.<\/p>\n<p>NOTE       Extraction and pre-concentration of water samples can prove necessary.<\/p>\n","protected":false},"excerpt":{"rendered":"<p><b>Water quality \u2014 Determination of the estrogenic potential of water and waste water \u2014 Part 2: Yeast estrogen screen (A-YES, Arxula adeninivorans)<\/b><\/p>\n<table style=\"width: 100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Published By<\/td>\n<td>Publication Date<\/td>\n<td>Number of Pages<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/pdfstandards.shop\/product-category\/publishers\/iso\/\"><b>ISO<\/b><\/a><\/td>\n<td>2018-08<\/td>\n<td>62<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":601871,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[218,2634],"product_tag":[],"class_list":{"0":"post-601858","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-13-060-70","7":"product_cat-iso","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/601858","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/601871"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=601858"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=601858"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=601858"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}