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ASTM-D5712:2005 Edition

ASTM-D5712:2005 Edition

$40.63

D5712-05e1 Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method

Published By Publication Date Number of Pages
ASTM 2005 9
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1.1 This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with natural rubber (NR) and its products. Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.

1.2 For the purpose of this test method, the range of protein measurement will be based on the limit of detection and quantitation and recorded in micrograms per dm 2 NR specimen.

1.3 The test method is designed to be accurate and compatible with the industrial environment.

1.4 Steps are included in this test method to minimize the effects of interfering substances.

1.5 It is recognized that other methods for the analysis of leachable proteins exist and these may be used for routine quality control purposes provided they have been validated and a correlation established against the reference method specified by this test method.

1.6 The values stated inSI units are to be regarded as the standard. No other units of measurement are included in this standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

PDF Catalog

PDF Pages PDF Title
1 Scope
Referenced Documents
Terminology
2 Summary of Test Method
Significance and Use
Apparatus
Reagents and Materials
3 Hazards
Extraction and Assay Procedures
5 Calculation
6 Report
Statistical Information: Precision and Bias, and Limit of Quantitation
FIG. 1
TABLE 1
7 Keywords
X1. AMINO ACID ANALYSIS OF NATURAL RUBBER PROTEINS
X1.1 Significance and Use
X1.2 Materials/Apparatus
X1.3 Product Extraction
X1.4 Hydrolysis
X1.5 Precolumn Derivatization with o-Phthaldialdehyde (OPA) and 3-Mercaptopropionic Acid (MPA)
TABLE X1.1
8 X1.6 HPLC Separation Conditions
X1.7 Calculations
TABLE X1.2
9 REFERENCES

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