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ASTM-E2721:2010 Edition

ASTM-E2721:2010 Edition

$40.63

E2721-10 Standard Test Method for Evaluation of Effectiveness of Decontamination Procedures for Surfaces When Challenged with Droplets Containing Human Pathogenic Viruses

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ASTM 2010 10
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1.1 This test method is designed to evaluate decontamination methods (physical, chemical, self-decontaminating materials) when used on surfaces contaminated with virus-containing droplets.

1.2 This test method defines the conditions for simulating respiratory droplets produced by humans and depositing the droplets onto surfaces.

1.3 The method is specific to influenza viruses but could be adapted for work with other types of respiratory viruses or surrogates (Appendix X5).

1.4 This test method is suitable for working with a wide variety of environmental surfaces.

1.5 This test method does not address the performance of decontaminants against microbes expelled via blood splatter, vomit, or fecal contamination.

1.6 This test method should be performed only by those trained in bioaerosols, microbiology, or virology, or combinations thereof.

1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

PDF Catalog

PDF Pages PDF Title
1 Scope
Referenced Documents
2 Terminology
Summary of Test Method
Significance and Use
Apparatus
3 Reagents and Materials
Sampling, Test Specimens, and Test Units
Experimental Design
Test Procedure
TABLE 1
4 Calculation or Interpretation of Results
5 Report
Precision and Bias
Keywords
X1. EXAMPLE DEVICE: OPERATION OF THE DROPLET CHAMBER TESTING SYSTEM
X1.1 Diagram of the Droplet Loader
X1.2 Parameters of the Droplet Loader
6 X1.3 Test Procedure
X2. TCID50 ASSAY FOR INFLUENZA
X2.1 Reagents
FIG. X1.1
7 X2.2 Method
X3. EXAMPLE CALCULATION FOR TISSUE CULTURE INFECTIOUS DOSE (TCID50) ASSAY
X3.1 24-Well Plate
X3.2 96-Well Plate
FIG. X3.1
FIG. X3.2
8 X4. EXAMPLE CALCULATION FOR DATA QUALITY OBJECTIVES AND DECONTAMINATION EFFICIENCY
X4.1 Example Data Set
X4.2 Average log10TCID50/cm2
X4.3 Data Quality Objectives
X4.4 Decontamination Efficiency
X5. METHOD ADAPTATION FOR USING BACTERIOPHAGE MS2
X5.1 Scope
X5.2 Referenced Documents
X5.3 Terminology
X5.4 Summary of Test Tethod
X5.5 Significance and Use
X5.6 Additional Equipment
X5.7 Additional Reagents and Materials
9 X5.8 Sampling, Test Specimens, and Test Units
X5.9 Experimental Design
X5.10 Test Procedure
X5.11 Calculation and Interpretation of Results
X5.12 Additional Reporting
X5.13 Precision and Bias
X5.14 Additional Keywords
X6. MS2 PLAQUE ASSAY
X6.1 Preparation of E. coli (Day 1)
X6.2 Inoculation of TSB With E. coli (Day 2)
X6.3 Media Preparation and Plating (Day 3)
X6.4 Prepare MS2 Soft-Agar Medium
X6.5 Plating of Samples

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