🔥🔥 Don’t Miss Out on Our End of Autumn Sale. If you have any questions, feel free to click the bottom right corner to contact our customer service..

Concat us[email protected]
Shopping Cart

No products in the cart.

🔍
BS EN 12353:2021

BS EN 12353:2021

$167.15

Chemical disinfectants and antiseptics. Preservation of test organisms used for the determination of bactericidal (including Legionella), mycobactericidal, sporicidal, fungicidal and virucidal (including bacteriophages) activity

Published By Publication Date Number of Pages
BSI 2021 40
PDF Format Searchable Printable Preview Now
Guaranteed Safe Checkout
Categories: ,

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. We’re here to assist you 24/7.
Email:[email protected]

This document specifies methods for keeping test organisms used and defined in European Standards for the determination of bactericidal (incl. Legionella pneumophila), mycobactericidal, sporicidal, fungicidal and virucidal (incl. bacteriophages) activity of chemical disinfectants and antiseptics drawn up by CEN/TC 216. These methods for keeping test organisms can only be carried out in connection with at least one of those standards where a reference to this document is established.

NOTE 1

Annex A (informative) contains a non-exhaustive list of test organisms for which this document can be applied.

NOTE 2

European Standards (EN) where this document is referenced are listed in the Bibliography.

NOTE 3

A specific description on the preservation of bacterial spores could be added once the results of the ongoing ring trials are available.

PDF Catalog

PDF Pages PDF Title
2 undefined
8 1 Scope
2 Normative references
3 Terms and definitions
4 Requirements
9 5 Methods
5.1 Principle
5.2 Test organisms, culture media and reagents
5.2.1 Test organisms
5.2.2 Culture media and reagents
5.2.2.1 General
5.2.2.2 Water
10 5.2.2.3 Tryptone Soya Broth (TSB) for bacteria, except Legionella
5.2.2.4 Malt Extract Broth (MEB) for fungi
5.2.2.5 Cryoprotectant solution for bacteria and fungi
11 5.2.2.6 Middlebrook 7 H 9 broth with 10 % ADC enrichment and glycerol as reconstituent and cryoprotectant solution for mycobacteria (MADC)
5.2.2.7 Polysorbate 80 solution
5.2.2.8 DMSO as cryoprotectant for cell culture freezing
5.2.2.9 Glutamine solution (3 % m/V solution)
5.2.2.10 TV (Trypsin-Versene)
5.2.2.11 Antibiotic suspension
12 5.2.2.12 Phosphate-buffered saline solution (PBS)
5.2.2.13 Fetal calf serum (FCS)
5.2.2.14 Earle’s BSS
13 5.2.2.15 Phenol red (1 % m/V solution)
5.2.2.16 Sodium bicarbonate (8,8 % w/v solution)
5.2.2.17 Cell culture medium
5.2.2.18 M17-broth
5.2.2.19 M17-agar (bottom agar)
14 5.2.2.20 Overlay agar (top agar, soft agar)
5.2.2.21 CaCl2-stock solutions (1 mol/l and 0,05 mol/l)
5.2.2.22 Reconstituted skim milk
5.2.2.23 Buffered Charcoal Yeast Extract (BCYE) Broth, for Legionella
15 5.2.2.24 Buffered Charcoal Yeast Extract (BCYE) Agar, for Legionella
5.2.2.25 Page’s saline (cryoprotective solution for Legionella)
16 5.2.3 Cell cultures
5.2.3.1 Storage of cell cultures
5.2.3.2 Preparation of new cell cultures
17 5.2.3.3 Passages
5.2.4 Host strains for dairy bacteriophages (Lactococcus lactis)
5.2.4.1 Stock culture of host bacteria
5.2.4.2 Working culture of host bacteria
18 5.3 Apparatus and glassware
5.3.1 General
5.3.2 Usual microbiological laboratory equipment
19 5.4 Procedure for preservation of test organisms – General
5.4.1 Handling of freeze-dried / frozen test organisms from culture collections
5.4.2 Choice of incubation procedure, agar medium, cell culture/cell line
20 5.5 Procedure for preservation of bacteria (incl. Legionella, aerobic spore-forming bacteria, excl. mycobacteria and bacterial spores) and yeasts
5.5.1 Reconstitution of the freeze-dried test organisms
5.5.2 Preparation for storage
21 5.5.3 Preparation of stock culture / working cultures
5.6 Procedure for preservation of mycobacteria
5.6.1 Reconstitution of the freeze-dried test organisms
5.6.2 Preparation for storage
22 5.6.3 Preparation of working cultures
5.7 Procedure for preservation of moulds (e.g. Aspergillus brasiliensis)
5.7.1 Reconstitution of the freeze-dried test organism
5.7.2 Preparation for storage
23 5.7.3 Preparation of stock culture / working cultures
5.8 Procedure for preservation of viruses (except dairy bacteriophages)
5.8.1 Reconstitution of frozen virus
5.8.2 Preparation for storage of stock virus suspension
5.8.3 Preparation of test virus suspension
24 5.9 Procedure for preservation of bacteriophages
5.9.1 Reconstitution of frozen bacteriophages
5.9.2 Preparation for storage
5.9.3 Preparation of bacteriophages working suspensions
5.10 Verification of the purity and identity of test organisms
5.10.1 General
25 5.10.2 Information on source of strains
5.10.3 Purity
5.10.4 Identity
5.11 Documentation
5.11.1 General
5.11.2 Freeze-dried test organism / frozen viruses
5.11.3 Cryovials of frozen test organism
26 5.11.4 Stock culture
5.11.5 Verification of purity and identity
5.11.6 Storage of documentation
27 Annex A (informative)Test organisms – Culture collection references and relation to CEN/TC 216 standards
A.1 Bacteria (except mycobacteria and spore-forming bacteria)
A.1.1 Enterobacter cloacae
A.1.2 Enterococcus hirae
A.1.3 Enterococcus faecium
A.1.4 Escherichia coli (1)
A.1.5 Escherichia coli (2) K12
A.1.6 Lactobacillus brevis
A.1.7 Legionella pneumophila, subsp. Pneumophila
A.1.8 Proteus hauseri
28 A.1.9 Pseudomonas aeruginosa
A.1.10 Salmonella enterica subsp. enterica, serotype Typhimurium
A.1.11 Staphylococcus aureus subsp. aureus
A.2 Mycobacteria
A.2.1 Mycobacterium avium subsp. avium
A.2.2 Mycobacterium terrae
A.3 Spore-forming bacteria
A.3.1 Bacillus cereus
A.3.2 Bacillus subtilis subsp. spizizenii
A.3.3 Clostridioides difficile (former “Clostridium difficile”) R027
29 A.3.4 Clostridium sporogenes
A.4 Fungi (moulds and yeasts)
A.4.1 Aspergillus brasiliensis (former “A. niger”) (mould)
A.4.2 Candida albicans (yeast)
A.4.3 Saccharomyces cerevisiae (1) (yeast)
A.4.4 Saccharomyces cerevisiae (2) var. diastaticus (yeast)
A.5 Viruses
A.5.1 Adenovirus type 5, strain Adenoid 75
A.5.2 Enterovirus E (Enterovirus Bovine Type 1), strain LCR 4 (former “Bovine enterovirus Type 1(ECBO)”)
30 A.5.3 Feline coronavirus (Alphacoronavirus 1), Strain Munich (FeCoV)
A.5.4 Minute virus of mice (Rodent protoparvovirus 1) (former “Murine parvovirus, minute virus of mice, strain Crawford”)
A.5.5 Murine norovirus, strain S99 Berlin
A.5.6 Poliovirus type 1, Sabin strain LSc-2ab
A.5.7 Porcine parvovirus (Ungulate protoparvovirus I), Strain NADL-2 (PPV)
A.5.8 Vaccinia virus, strain modified vaccinia virus Ankara (MVA)
A.5.9 Vaccinia virus, strain Elstree
A.6 Bacteriophages
A.6.1 Lactococcus lactis subsp. lactis bacteriophage P008
A.6.2 Lactococcus lactis subsp. lactis bacteriophage P001
31 Annex B (informative)Graphical representations

Related products

BS EN 12353:2021
$167.15