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BS EN 12823-1:2014

BS EN 12823-1:2014

$102.76

Foodstuffs. Determination of vitamin A by high performance liquid chromatography – Measurement of all-E-retinol and 13-Z-retinol

Published By Publication Date Number of Pages
BSI 2014 20
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This European Standard specifies a method for the determination of vitamin A in foodstuffs by high performance liquid chromatography (HPLC). This method has been validated in an interlaboratory study with samples of margarine and milk powder with all-E-retinol levels ranging from 653 µg/100 g to 729 µg/100 g and with 13-Z-retinol levels ranging from 30 µg/100 g to 39 µg/100 g. The determination of vitamin A content is carried out by the measurement of all-E-retinol, 13-Z-retinol and β-carotene. This part covers the measurement of all-E-retinol and 13-Z-retinol.

The extract obtained after saponification in this method can be used for the determination of β-carotene, as described in EN 12823-2:2000, Foodstuffs – Determination of vitamin A by high performance liquid chromatography – Part 2: Measurements of β-carotene. In this case, the saponification temperature should preferably not exceed 80 °C in order to prevent isomerisation and oxidation of β-carotene.

PDF Catalog

PDF Pages PDF Title
4 Contents Page
5 Foreword
6 1 Scope
2 Normative references
3 Principle
4 Reagents
9 5 Apparatus
10 6 Procedure
6.1 General
6.2 Preparation of the test sample
6.3 Preparation of the sample test solution
6.3.1 Saponification
Table 1 — Examples of suitable ratios of reagents
11 6.3.2 Extraction
6.3.3 Evaporation
6.3.4 Dilution
6.4 Preparation of the calibration solutions
6.5 Identification and purity check
12 6.6 Determination
7 Calculation
13 8 Precision
8.1 General
8.2 Repeatability
14 8.3 Reproducibility
9 Test report
15 Annex A (informative) Examples of HPLC chromatograms
Figure A.1 — HPLC chromatograms of vitamin A standards (a), vitamin A in margarine (b) and milk powder (c)
16 Annex B (informative) Precision data
Table B.1 — Precision data for margarine and milk powder
17 Annex C (informative) Alternative HPLC systems
Table C.1 — Alternative HPLC systems and chromatographic conditions
18 Bibliography

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BS EN 12823-1:2014
$102.76