🔥🔥 Don’t Miss Out on Our End of Autumn Sale. If you have any questions, feel free to click the bottom right corner to contact our customer service..

Concat us[email protected]
Shopping Cart

No products in the cart.

🔍
BS EN 17641:2022

BS EN 17641:2022

$198.66

Foodstuffs. Multimethod for the determination of aflatoxins, deoxynivalenol, fumonisins, ochratoxin A, T-2 toxin, HT-2 toxin and zearalenone by LC-MS/MS

Published By Publication Date Number of Pages
BSI 2022 52
PDF Format Searchable Printable Preview Now
Guaranteed Safe Checkout
Category:

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. We’re here to assist you 24/7.
Email:[email protected]

This document describes a method using isotopically labelled standards for the quantitative determination of aflatoxins B1, B2, G1, G2 and M1 (AFB1, AFB2, AFG1, AFG2 and AFM1), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN), T-2 and HT-2 toxins (T-2 and HT-2) and fumonisins B1 and B2 (FB1 and FB2) in foods by liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS). A specific immunoaffinity column (IAC) clean-up is needed for aflatoxins (AFs) and OTA in food intended for infants and young children (e.g. infant cereals, milk-based powders), in spices, in dried fruits and in nuts. The method has been validated through an intercollaborative study on different commodity groups: cereals and cereal-based products including food for infant and young children, nuts, spices, dried fruits and milk powder. The measuring range of each mycotoxin in these naturally contaminated and/or spiked food samples were: – AFB1: 0,085 7 µg/kg – 11,4 µg/kg; – AFB2: 0,079 2 µg/kg – 12,5 µg/kg; – AFG1: 0,062 8 µg/kg – 20,9 µg/kg; – AFG2: 0,052 0 µg/kg – 15,0 µg/kg; – AFM1: 0,034 2 µg/kg – 0,110 µg/kg; – OTA: 0,448 µg/kg – 17,2 µg/kg; – DON: 45,2 µg/kg – 743 µg/kg; – ZEN: 9,57 µg/kg – 131 µg/kg; – T-2: 10,3 µg/kg – 57,9 µg/kg; – HT-2: 9,50 µg/kg – 81,8 µg/kg; – FB1: 31,1 µg/kg – 4 260 µg/kg; – FB2: 44,2 µg/kg – 1 300 µg/kg. The measuring ranges of the method for each mycotoxin/matrix combination are given in Table 8.

PDF Catalog

PDF Pages PDF Title
2 undefined
7 1 Scope
2 Normative references
3 Terms and definitions
8 4 Principles
5 Reagents
14 6 Apparatus and equipment
16 7 Procedure
7.1 Preparation of the laboratory sample
7.2 Test portions weighing
7.2.1 Cereals, cereal-based products
7.2.2 Milk powders, nuts, spices, dried fruits
7.3 Spiking with ISTD solutions
7.4 Extraction
7.4.1 General procedure
17 7.4.2 Without IAC clean-up
7.4.3 With IAC clean-up
18 8 LC-MS/MS analysis
8.1 Operating conditions
8.2 Injection sequence
19 8.3 Data treatment
9 Calculations
9.1 Identification and confirmation
9.2 Calibration curve and measuring range
20 9.3 Calculations
21 10 Precision
10.1 General
10.2 Repeatability
10.3 Reproducibility
24 11 Test report
25 Annex A (informative)Precision data
41 Annex B (informative)Overview of the sample preparation
42 Annex C (informative)Example conditions for suitable LC-MS/MS systems
C.1 General
C.2 LC system settings examples
C.2.1 LC conditions 1
43 C.2.2 LC conditions 2
C.2.3 LC conditions 3
44 C.2.4 LC conditions 4
45 C.2.5 LC conditions 5
C.3 MS system settings examples
C.3.1 MS conditions 1
47 C.3.2 MS conditions 2
49 C.3.3 MS conditions 3

Related products

BS EN 17641:2022
$198.66