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BS EN 17658:2022

$198.66

Chemical disinfectants and antiseptics. Chemical textile disinfection for the domestic area. Test method and requirements (phase 2, step 2)

Published By Publication Date Number of Pages
BSI 2022 52
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This document specifies a test method and the minimum performance requirements for the microbicidal efficacy of a chemical product intended for use in a wash process in a domestic environment, in a domestic wash equipment at low temperatures (≤40 °C). This procedure does not apply to certain types of laundry disinfection technologies which require specific devices (i.e. active substances generated in situ using specific devices). This method is not limited to certain types of textiles, types of products or steps in the washing cycle. According to a phase 2, step 2 test definition, this document establishes the efficacy in laboratory test simulating practical use conditions of a chemical product. This document cannot be applied when the disinfection is medical indicated (medical area) or in hygiene-sensitive areas where professional reprocessing of laundry is required (i.e. food, healthcare, medical and cleanroom sectors, PPE, and workwear). In those cases, EN 16616 and EN 14065 will apply. NOTE This method corresponds to a phase 2, step 2 test (see EN 14885). EN 14885 specifies in detail the relationship of the various tests to one another and to “use recommendations”.

PDF Catalog

PDF Pages PDF Title
2 undefined
8 1 Scope
2 Normative references
3 Terms and definitions
9 4 Requirements
11 5 Test method
5.1 Principle
5.2 Materials and reagents
5.2.1 Test organisms
5.2.2 Culture media and reagents
5.2.2.1 General
12 5.2.2.2 Water used for preparation of media
5.2.2.3 Water of standardized hardness
5.2.2.4 Tryptone Soy Agar (TSA)
13 5.2.2.5 Malt Extract Agar (MEA)
5.2.2.6 Diluent
5.2.2.7 Neutralizer
5.2.2.8 Interfering substance
5.3 Apparatus and glassware
5.3.1 General
16 5.4 Preparation of test organism suspensions
5.4.1 Test organism suspensions (test suspension)
5.4.1.1 General
5.4.1.2 Preservation and stock cultures of test organisms
5.4.1.3 Working culture and test organisms
17 5.4.1.4 Test suspension (N)
18 5.4.1.5 Inoculation of the carriers
5.5 Procedure for assessing the microbicidal activity of the product
5.5.1 General
5.5.1.1 Experimental conditions (obligatory and additional)
5.5.1.2 Choice of neutralizer
19 5.5.1.3 General instructions for validation and control procedures
5.5.1.4 Precautions for manipulation of test organisms
5.5.2 Method
5.5.2.1 General
5.5.2.2 Canister preparation
5.5.2.3 Preparation of the testing canister
21 5.5.2.4 Preparation of test product dilutions
5.5.2.5 Test procedure ‘Na’ (Determination of microbicidal concentrations) and reference ‘N0’ (Microbial content on carriers)
22 5.5.2.6 Reference control ‘NW’ (Test without product)
5.5.2.7 Control ‘RI’ (Non contaminated cotton carrier in the test procedure and the reference control)
5.5.2.8 Control ‘WW’ (wash water counts in the test procedure and the reference control)
23 5.5.2.9 Incubation and counting
5.6 Experimental data and calculation
5.6.1 Explanation of terms and abbreviations
5.6.1.1 Overview of the different suspensions and test mixtures
5.6.1.2 VC-values
5.6.2 Calculation
5.6.2.1 General
24 5.6.2.2 Determination of VC-counts
5.6.2.3 Calculation of N and N0
25 5.6.2.4 Calculation of Na
5.6.2.5 Calculation of NW
26 5.6.2.6 Calculation of RI
5.6.2.7 Calculation of WW
27 5.7 Verification of methodology
5.7.1 General
5.7.2 Control of weighted mean counts
5.7.3 Basic limits
28 5.8 Expression of results and precision
5.8.1 Reduction
5.8.2 Repetitions
29 5.9 Interpretation of results – conclusion
5.9.1 General
5.9.2 Microbicidal activity
5.9.2.1 Main wash cycle
30 5.9.2.2 Rinse cycle
32 5.10 Test report
34 Annex A (informative)Referenced strains in national collections
36 Annex B (informative)Suitable neutralizers and rinsing liquids
B.1 General
B.2 Neutralizers
37 B.3 Neutralizer added to the agar for counting
38 Annex C (informative)Graphical representations of the test method
40 Annex D (informative)Example of lab-scale devices
41 Annex E (informative)Test report (example)
48 Annex F (informative)SBL2004 composition
49 Annex G (informative)Stainers and markers validated
BS EN 17658:2022
$198.66