BS EN 17658:2022
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Chemical disinfectants and antiseptics. Chemical textile disinfection for the domestic area. Test method and requirements (phase 2, step 2)
Published By | Publication Date | Number of Pages |
BSI | 2022 | 52 |
This document specifies a test method and the minimum performance requirements for the microbicidal efficacy of a chemical product intended for use in a wash process in a domestic environment, in a domestic wash equipment at low temperatures (≤40 °C). This procedure does not apply to certain types of laundry disinfection technologies which require specific devices (i.e. active substances generated in situ using specific devices). This method is not limited to certain types of textiles, types of products or steps in the washing cycle. According to a phase 2, step 2 test definition, this document establishes the efficacy in laboratory test simulating practical use conditions of a chemical product. This document cannot be applied when the disinfection is medical indicated (medical area) or in hygiene-sensitive areas where professional reprocessing of laundry is required (i.e. food, healthcare, medical and cleanroom sectors, PPE, and workwear). In those cases, EN 16616 and EN 14065 will apply. NOTE This method corresponds to a phase 2, step 2 test (see EN 14885). EN 14885 specifies in detail the relationship of the various tests to one another and to “use recommendations”.
PDF Catalog
PDF Pages | PDF Title |
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2 | undefined |
8 | 1 Scope 2 Normative references 3 Terms and definitions |
9 | 4 Requirements |
11 | 5 Test method 5.1 Principle 5.2 Materials and reagents 5.2.1 Test organisms 5.2.2 Culture media and reagents 5.2.2.1 General |
12 | 5.2.2.2 Water used for preparation of media 5.2.2.3 Water of standardized hardness 5.2.2.4 Tryptone Soy Agar (TSA) |
13 | 5.2.2.5 Malt Extract Agar (MEA) 5.2.2.6 Diluent 5.2.2.7 Neutralizer 5.2.2.8 Interfering substance 5.3 Apparatus and glassware 5.3.1 General |
16 | 5.4 Preparation of test organism suspensions 5.4.1 Test organism suspensions (test suspension) 5.4.1.1 General 5.4.1.2 Preservation and stock cultures of test organisms 5.4.1.3 Working culture and test organisms |
17 | 5.4.1.4 Test suspension (N) |
18 | 5.4.1.5 Inoculation of the carriers 5.5 Procedure for assessing the microbicidal activity of the product 5.5.1 General 5.5.1.1 Experimental conditions (obligatory and additional) 5.5.1.2 Choice of neutralizer |
19 | 5.5.1.3 General instructions for validation and control procedures 5.5.1.4 Precautions for manipulation of test organisms 5.5.2 Method 5.5.2.1 General 5.5.2.2 Canister preparation 5.5.2.3 Preparation of the testing canister |
21 | 5.5.2.4 Preparation of test product dilutions 5.5.2.5 Test procedure ‘Na’ (Determination of microbicidal concentrations) and reference ‘N0’ (Microbial content on carriers) |
22 | 5.5.2.6 Reference control ‘NW’ (Test without product) 5.5.2.7 Control ‘RI’ (Non contaminated cotton carrier in the test procedure and the reference control) 5.5.2.8 Control ‘WW’ (wash water counts in the test procedure and the reference control) |
23 | 5.5.2.9 Incubation and counting 5.6 Experimental data and calculation 5.6.1 Explanation of terms and abbreviations 5.6.1.1 Overview of the different suspensions and test mixtures 5.6.1.2 VC-values 5.6.2 Calculation 5.6.2.1 General |
24 | 5.6.2.2 Determination of VC-counts 5.6.2.3 Calculation of N and N0 |
25 | 5.6.2.4 Calculation of Na 5.6.2.5 Calculation of NW |
26 | 5.6.2.6 Calculation of RI 5.6.2.7 Calculation of WW |
27 | 5.7 Verification of methodology 5.7.1 General 5.7.2 Control of weighted mean counts 5.7.3 Basic limits |
28 | 5.8 Expression of results and precision 5.8.1 Reduction 5.8.2 Repetitions |
29 | 5.9 Interpretation of results – conclusion 5.9.1 General 5.9.2 Microbicidal activity 5.9.2.1 Main wash cycle |
30 | 5.9.2.2 Rinse cycle |
32 | 5.10 Test report |
34 | Annex A (informative)Referenced strains in national collections |
36 | Annex B (informative)Suitable neutralizers and rinsing liquids B.1 General B.2 Neutralizers |
37 | B.3 Neutralizer added to the agar for counting |
38 | Annex C (informative)Graphical representations of the test method |
40 | Annex D (informative)Example of lab-scale devices |
41 | Annex E (informative)Test report (example) |
48 | Annex F (informative)SBL2004 composition |
49 | Annex G (informative)Stainers and markers validated |