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BS EN ISO 12966-4:2015

BS EN ISO 12966-4:2015

$142.49

Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters – Determination by capillary gas chromatography

Published By Publication Date Number of Pages
BSI 2015 30
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ISO 12966 specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). Fatty acid methyl esters from C8 to C24 can be separated using this part of ISO 12966 including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters.

The method is applicable to crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and fatty acids derived from animal and vegetable sources.

This method is not suitable for the analysis of dairy, ruminant fats and oils, or products supplemented with conjugated linoleic acid (CLA). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 12966 .

This part of ISO 12966 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils.

PDF Catalog

PDF Pages PDF Title
6 Foreword
7 1 Scope
2 Normative references
3 Principle
4 Reagents and materials
8 4.1 Reference fatty acid methyl esters (FAMEs)
4.2 Internal standards
9 5 Apparatus
10 6 Sampling
7 Preparation of test sample
8 Preparation of methyl esters from fats, oils, and fatty acids
9 Procedure
9.1 General
9.2 GC conditions
11 9.3 Performance check
10 Calculations
10.1 Qualitative analysis and peak identification
10.2 Quantitative analysis
10.2.1 Calculation of the composition of fatty acid methyl esters
12 10.2.2 Calculation of the composition of fatty acid methyl esters using correction factors
10.2.3 Calculation of the composition of fatty acid methyl esters using an internal standard
13 11 Precision
11.1 Results of interlaboratory test
11.2 Repeatability
11.3 Reproducibility
12 Test report
15 AnnexĀ A (informative) Theoretical flame ionization detector correction factor (TCF) for fatty acid methyl esters (FAMEs)
16 AnnexĀ B (informative) Examples of chromatograms
18 AnnexĀ C (informative) Comparison of FAME composition with two different GC columns
20 AnnexĀ D (informative) Results of an interlaboratory trial
26 Bibliography

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BS EN ISO 12966-4:2015
$142.49