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BS EN ISO 22753:2022

BS EN ISO 22753:2022

$167.15

Molecular biomarker analysis. Method for the statistical evaluation of analytical results obtained in testing sub-sampled groups of genetically modified seeds and grains. General requirements

Published By Publication Date Number of Pages
BSI 2022 38
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This document describes general requirements, procedures and performance criteria for evaluating the content of genetically modified (GM) seeds/grains in a lot by a group testing strategy that includes qualitative analysis of sub-sampled groups followed by statistical evaluation of the results. This document is applicable to group testing strategy estimating the GM content on a percentage seed/grain basis for purity estimation, testing towards a given reject/accept criterion and for cases where seed/grain lots are carrying stacked events. This document is not applicable to processed products. NOTE       Description of the use of group testing strategy are available in References [1], [7], [8], [18], [19] and [20].

PDF Catalog

PDF Pages PDF Title
2 undefined
4 European foreword
Endorsement notice
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
12 4 Principle
4.1 General
4.2 Preparation of seed/grain groups
13 4.3 Detection methods for the qualitative analysis of GM seed/grain in seed/grain groups
4.4 Statistical evaluation
14 5 Reagents
6 Apparatus and equipment
7 Design of testing plan
7.1 General
7.2 Single-stage testing plan
15 7.3 Double-stage testing plan
16 8 Selection of qualitative methods
8.1 General
8.2 Performance criteria
9 Interpretation
18 10 Expression of results
10.1 Classification of a seed/grain lot into “accept” or “reject” category
10.2 Estimation of the level of molecular biomarker in the seed/grain lot
11 Test report
20 Annex A (informative) Terms and definitions comparison table
22 Annex B (informative) Implementation of the method to evaluate GMO content in seeds/grains example
29 Annex C (informative) Estimation of the limit of detection for a testing plan to detect GM seeds/grains in seed lots
32 Annex D (informative) Experimental determination of maximum group size
33 Bibliography

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BS EN ISO 22753:2022
$167.15