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BS EN ISO 23036-2:2021

BS EN ISO 23036-2:2021

$102.76

Microbiology of the food chain. Methods for the detection of Anisakidae L3 larvae in fish and fishery products – Artificial digestion method

Published By Publication Date Number of Pages
BSI 2021 18
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This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or smoked. It is also suitable for visceral organs as a confirmatory method for a visual inspection scheme.

The artificial digestion method[ 4][ 5][ 6] is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature and, when applied to fresh fish or lightly processed fish products (never frozen before processing), determining the viability of Anisakidae L3, which can be present.

This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
4.1 General
4.2 Sample size
4.3 Sample preparation
4.4 Digestion of the sample
4.5 Filtration of the digest fluid
11 4.6 ​Verification of findings
5 Reagents
5.1 Tap water.
5.4 90 % ethanol.
6 Equipment and consumables
12 7 Sampling
8 Procedure
8.1 Preparation of the sample
8.2 Preparation of the digest fluid
13 8.3 Digestion of the sample in the glass beaker
8.4 Filtration of the digest fluid
8.5 Microscopic examination
14 9 Expression of the results
10 Performance characteristics of the method
11 Test report
12 Quality assurance
15 Annex A (informative) Sample collection
16 Annex B (informative) Example of a laboratory worksheet for recording data when testing fish fillets with the artificial digestion method
17 Bibliography

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BS EN ISO 23036-2:2021
$102.76