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BS EN ISO 4973:2023

BS EN ISO 4973:2023

$142.49

Cosmetics. Microbiology. Quality control of culture media and diluents used in cosmetics standards

Published By Publication Date Number of Pages
BSI 2023 30
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This document specifies the minimum requirements for quality control of microbiological culture media and diluents in order to demonstrate their ability to detect microorganisms and to ensure reliability of the microbiological test methods described in the ISO cosmetics microbiology standards. This document describes mainly growth promotion and microbial control tests and is applicable to both commercially ready-to-use culture media and culture media prepared from dehydrated culture media or basic constituents in the user’s laboratory. Other methods can be substituted provided that their equivalence has been demonstrated.

PDF Catalog

PDF Pages PDF Title
2 undefined
4 European foreword
Endorsement notice
7 Foreword
8 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
11 4 Principle
4.1 General information
4.2 pH
4.3 Absence of microbiological growth
4.4 Growth promotion
4.5 Selective and indicative properties
12 5 Diluents, neutralizers and culture media
5.1 General
5.2 Diluents and neutralizers
5.3 Culture media
6 Apparatus and glassware
7 Strains of microorganisms
14 8 Procedure
8.1 General recommendation
8.2 Preparation of strains
8.2.1 General
17 8.2.2 Preparation of bacterial and Candida albicans suspensions
8.2.3 Preparation of Aspergillus brasiliensis spore stock suspension
18 8.2.4 Control of the concentration of the calibrated suspension
8.3 Absence of microbial growth
8.3.1 Solid culture media
8.3.2 Liquid culture media and diluents
8.4 Growth promotion
8.4.1 Solid culture media
19 8.4.2 Liquid culture media
8.5 Selective properties
8.5.1 Solid culture media — For indicative properties
8.5.2 Solid culture media — For inhibition properties
9 Expression of results
9.1 Absence of microbial growth
9.1.1 Solid culture media
9.1.2 Liquid culture media
9.2 Growth promotion
9.2.1 Solid culture media
9.2.2 Liquid culture media
20 9.3 Selective and indicative properties
9.3.1 Solid culture media — For indicative properties
9.3.2 Solid culture media — For inhibition properties
10 Interpretation and acceptance criteria
10.1 General
10.2 Absence of microbial growth
10.3 Growth promotion
10.3.1 Solid culture media
21 10.3.2 Growth promotion — Liquid media culture
10.4 Selective properties of solid culture media
10.4.1 For indicative properties
10.4.2 For inhibition properties
22 Annex A (normative) Specifications for growth promoting, inhibitory and indicative properties of culture media
27 Annex B (informative) Quality control report for culture media
29 Bibliography

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BS EN ISO 4973:2023
$142.49