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BS ISO 15163:2012

BS ISO 15163:2012

$142.49

Milk and milk products. Calf rennet and adult bovine rennet. Determination by chromatography of chymosin and bovine pepsin contents

Published By Publication Date Number of Pages
BSI 2012 30
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PDF Catalog

PDF Pages PDF Title
9 1 Scope
2 Normative references
3 Principle
10 4 Reagents
11 5 Apparatus
12 6 Sampling
7 Procedure
7.1 Check
7.2 Preparation of a fresh column with Fractogel
7.3 Regeneration and equilibration of the Fractogel resin in the column
13 7.4 Storage of the column with Fractogel
7.5 Preparation of test sample
7.5.1 General
7.5.2 Desalting by dialysis
7.5.3 Desalting by gel filtration
14 7.5.4 Column lifetime
7.6 Analysis of the desalted rennet
7.6.1 Separation of chymosin and pepsin by ordinary column — Manual set-up
7.6.2 Separation of chymosin and pepsin by FPLC®/ÄKTA®/HPLC — Automatic set-up
15 7.7 Determination of the clotting times
16 8 Calculation and expression of results
8.1 Calculation of the activity of chymosin and pepsin, expressed as a percentage
17 8.2 Calculation of active chymosin and active bovine pepsin, in milligrams per litre
18 8.3 Expression of results
9 Precision
9.1 Interlaboratory test
9.2 Repeatability
19 9.3 Reproducibility
10 Test report

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BS ISO 15163:2012
$142.49