BS ISO 21470:2020
$167.15
Infant formula and adult nutritionals. Simultaneous determination of total vitamins B1, B2, B3 and B6. Enzymatic digestion and LC-MS/MS
Published By | Publication Date | Number of Pages |
BSI | 2020 | 38 |
This document specifies a method for the simultaneous quantitative determination of four water-soluble vitamins in infant formula and related nutritional products, including relevant forms of vitamins B1, B2, B3 and B6 by enzymatic digestion and UHPLC-MS/MS. This document is not intended to be used on products where vitamins have not been added.
PDF Catalog
PDF Pages | PDF Title |
---|---|
2 | undefined |
6 | Foreword |
7 | 1 Scope 2 Normative references 3 Terms and definitions 4 Principle |
8 | 5 Reagents and materials |
9 | 6 Standard and solution preparation 6.1 Mobile phases and prepared solutions |
10 | 6.2 Stable isotope labelled compounds, individual, internal standard stock solutions |
11 | 6.3 Stock standard solutions of native compounds |
12 | 6.4 Working standard solution preparation |
13 | 6.5 Summary of standard and solution preparation 7 Apparatus |
15 | 8 Procedure 8.1 Sample preparation 8.1.1 Powdered products 8.1.2 Reconstituted powders and liquid products 8.2 Enzymatic digestion 8.3 UHPLC-MS/MS analysis 8.3.1 UHPLC conditions |
16 | 8.3.2 MS tune conditions 8.3.3 Mass transitions |
17 | 8.3.4 LC-MS/MS equilibration 8.4 Quality control 8.4.1 General 8.4.2 Calibration curve 9 Calculations |
19 | 10 Precision data 10.1 General 10.2 Repeatability 10.3 Reproducibility |
21 | 11 Test report |
22 | Annex A (informative) Precision data |
31 | Annex B (informative) Comparison between this document and EN 14122 |
33 | Annex C (informative) Comparison between this document and EN 14152 |
35 | Annex D (informative) Comparison between this document and EN 14164 |
37 | Bibliography |