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BS ISO 23040:2021:2022 Edition

BS ISO 23040:2021:2022 Edition

$215.11

Marine environment impact assessment (MEIA). Specification for marine sediments in seabed areas. Survey of interstitial biota

Published By Publication Date Number of Pages
BSI 2022 116
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PDF Catalog

PDF Pages PDF Title
2 National foreword
10 Foreword
11 Introduction
13 1 Scope
2 Normative references
3 Terms and definitions
14 4 General
4.1 Technical design
4.2 Basic recommendations for the surveys
4.2.1 Survey object
15 4.2.2 Auxiliary parameters
4.2.3 Recommendations for the sampling equipment
4.2.4 Auxiliary equipment on board ship
4.2.5 Sampling method and scope of application
4.3 Sampling
4.3.1 Sediment sampling
4.3.2 Trawl sampling
16 4.3.3 Water sampling
4.3.4 Records
4.4 Sample analysis
4.4.1 Sample treatment
4.4.2 Sample measurement
4.4.3 Sample treatment and storage
4.4.4 Sample identification and enumeration
4.4.5 Sample data analysis
17 4.5 Basic recommendations of data organization
4.5.1 Organization of data
4.5.2 Data archiving, acceptance and achievements appraisal
4.6 Survey results
4.6.1 Original records
4.6.2 Maps or drawings
4.6.3 Investigation report
18 4.7 Data archiving
4.8 Program and quality control
5 Survey of the sediment
5.1 Principle
19 5.2 General provisions
5.3 Collection and preservation of the samples
5.4 Measurement of environmental factors
5.5 Measurement of the age of the sediments
5.6 Measurement of the contents of heavy metals, organic pollutants and oils in the sediments
5.7 Measurement of the grain size of the sediments
20 5.8 Analysis of the mineral compositions in the sediments
5.9 Classification of the substrate type in offshore sediments
5.10 Classification of the deep-sea sediments
5.11 Survey of the biological components in the sediments
5.11.1 Summary of the methods
5.11.2 Technical recommendations
21 5.11.3 Collection and preservation of the samples
5.12 Organization of data
6 Survey of foraminifera
6.1 Principle
6.2 General provisions
6.3 Collection and preservation of the samples
6.3.1 Sampling and sample processing
22 6.3.2 Collection and preservation of the sedimentary tests
6.3.3 Collection and preservation of living foraminifera
6.4 Tools and reagents
6.5 Processing and analysis of the samples
6.5.1 Numbering and weighing the crystallizing dish or beaker
23 6.5.2 Drying and weighing the sediment sample
6.5.3 Sample soaking
6.5.4 Washing and drying
6.5.5 Suspension and concentration
6.5.6 Bottling and sealing
6.5.7 Specimen preparation and analysis
24 7 Survey of ostracoda
7.1 Principle
7.2 General provisions
7.3 Collection and preservation of the samples
7.3.1 Sampling and sample processing
25 7.3.2 Collection and preservation of dead ostracod tests
7.3.3 Collection and preservation of living ostracods
7.4 Tools and reagents
7.5 Processing and analysis of the samples
7.5.1 Numbering and weighing the crystallizing dish or beaker
7.5.2 Drying and weighing
26 7.5.3 Sample soaking
7.5.4 Washing and drying
7.5.5 Suspension and concentration
7.5.6 Bottling and sealing
7.5.7 Microscopic examination and enumeration
27 8 Survey of radiolaria
8.1 Principle
8.2 General provisions
8.3 Collection and preservation of the samples
8.3.1 Sample processing
28 8.3.2 Collection and conservation of deposited empty shells
8.4 Tools and reagents
8.5 Processing and analysis of the samples
8.5.1 Sample pretreatment
8.5.2 Preparation of microscope slide specimens
29 9 Survey of sedimentary diatom
9.1 Principle
9.2 General provisions
9.3 Collection and preservation of the samples
9.3.1 Sampling and treatment
30 9.3.2 Collection and preservation of sedimentary remains
9.4 Tools and reagents
9.5 Processing and analysis of the samples
9.5.1 Sample pretreatment
31 9.5.2 Preparation of microscope slide specimens
9.6 Organization of data
10 Survey of coccoliths
10.1 Principle
10.2 General provisions
32 10.3 Collection and preservation of the samples
10.4 Tools and reagents
10.5 Processing and analysis of the samples
10.5.1 Pre-preparation of the equipment and the samples
10.5.2 Method of preparing simple smear slides
33 10.5.3 Pre-concentration of coccolith samples
10.6 Organization of data
34 11 Survey of sporopollen
11.1 Principle
11.2 General provisions
35 11.3 Collection and preservation of the samples
11.4 Tools and reagents
11.5 Processing and analysis of the samples
11.5.1 Sample disaggregation
11.5.2 Alkali-soluble digestion
11.5.3 Sieving
36 11.5.4 Carbonate digestion
11.5.5 Silicate digestion
11.5.6 Ultrasonic cleaning and sieving
11.5.7 Acetolysis
37 11.5.8 Storage of processed sample
11.5.9 Mounting sporopollen specimens
11.5.10 Glycerine jelly preparation
11.5.11 Precautions
11.6 Organization of data
38 12 Survey of benthic viruses
12.1 Principle
12.2 General provisions
12.3 Collection and preservation of the samples
12.3.1 Samples for epifluorescence microscopy
12.3.2 Samples for flow cytometry
39 12.3.3 Samples for environmental measurements and molecular diversity of viruses
12.4 Tools and reagents
12.4.1 Equipment and reagents for epifluorescence microscopy
12.4.2 Equipment and reagents for flow cytometry
12.4.3 Equipment and tools for molecular diversity of viruses
40 12.5 Processing and analysis of samples
12.5.1 Enumeration of viruses by epifluorescence microscopy
41 12.5.2 Procedure for flow cytometry
42 12.5.3 Estimation of the molecular diversity of viruses
44 13 Survey of benthic microbes
13.1 Principle
13.2 General provisions
13.3 Collection and preservation of the samples
13.3.1 Sampling stations and sample collection
45 13.3.2 On-site sample process
13.3.3 Sample storage
13.4 Tools and reagents
13.4.1 Equipment
46 13.4.2 Reagents
48 13.5 Processing and analysis of the samples
13.5.1 Assessment of molecular diversities of benthic microbes
53 13.5.2 Benthic microbial abundance
56 14 Survey of benthic microalgae
14.1 Principle
14.2 General provisions
14.3 Collection and preservation of the samples
14.3.1 Sampling design
57 14.3.2 Sampling methods
14.3.3 Sample preservation and fixation
58 14.4 Tools and reagents
14.5 Processing and analysis of the samples
14.5.1 Sample processing
59 14.5.2 Sample analysis
60 14.5.3 Abundance and biomass calculation
61 14.5.4 Cyst culture and identification
62 14.5.5 Fluorescence determination of demagnesium chlorophyll and chlorophyll a
14.5.6 Algal toxins determination
63 15 Survey of benthic protozoa
15.1 Principle
15.2 General provisions
15.3 Collection and preservation of the samples
15.3.1 Sampling equipment
64 15.3.2 Sediment core collection
15.3.3 Sediment layers
15.3.4 Sample fixation and preservation
15.3.5 Collection and treatment of living protozoa
65 15.4 Tools and reagents
15.4.1 Equipment and reagents for silica sol centrifugation
66 15.4.2 Equipment and reagents of quantitative protargol staining
15.4.3 Equipment and reagents for molecular analysis
67 15.5 Processing and analysis of the samples
15.5.1 Silica sol density centrifugation
68 15.5.2 Quantitative protargol stain (QPS)
71 15.5.3 Estimation of molecular diversity
76 16 Survey of metazoan meiobenthos
16.1 Principle
16.2 General provisions
77 16.3 Collection and preservation of the samples
16.3.1 Sampling in intertidal zones and shallow waters
16.3.2 Deep-sea sampling
78 16.3.3 Sample slicing
16.3.4 Sample fixation and preservation
79 16.3.5 Labelling
16.3.6 Record
16.4 Tools and reagents
16.5 Processing and analysis of the samples
16.5.1 Quantification and taxa analysis
80 16.5.2 Molecular analysis of biodiversity
81 16.6 Organization of data
16.6.1 Precision
16.6.2 Abundance
82 16.6.3 Biomass
83 16.6.4 Complete the report
16.6.5 Plotting data
84 Annex A (informative) Photographs of several groups of communities of interstitial biota
86 Annex B (informative) Several stratified sampling devices for the survey of interstitial biota
88 Annex C (informative) Tables for sample labelling and sampling record — Examples
90 Annex D (informative) Tables of microscopy records — Examples
95 Annex E (informative) Calculation of community parameters
98 Annex F (informative) Record sheet for sample treatment — Examples
99 Annex G (informative) Characteristics for the classification of coccoliths
100 Annex H (informative) Flow diagram for the enumeration of viruses by epifluorescence microscopy
101 Annex I (informative) Filter unit used in microbenthos survey
102 Annex J (informative) Technical flowcharts for the investigation of microbenthos
105 Annex K (informative) Solutions and regents
111 Bibliography

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