BS ISO 4768:2023

$102.76

Measurement method of anti-biofilm activity on plastic and other non-porous surface

Published By	Publication Date	Number of Pages
BSI	2023	20

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Categories: 83.080.01 - Plastics in general, BSI

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Description

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PDF Pages	PDF Title
2	National foreword
6	Foreword
7	Introduction
9	1 Scope 2 Normative references 3 Terms and definitions 4 Materials 4.1 Bacterial strain to be used for the tests
10	4.2 Reagents 4.3 Culture media and solution 4.3.1 General 4.3.2 Nutrient agar (NA) 4.3.3 Suspension medium-1/5 Tryptic soy broth (1/5TSB) 4.3.4 Plate count agar 4.3.5 Slant culture medium 4.3.6 0,1 % Crystal violet aqueous solution
11	4.3.7 1,0 % Sodium dodecyl sulfate aqueous solution 5 Apparatus
12	6 Sterilization of apparatus and storage of stock cultures 6.1 Dry-heat sterilization 6.2 High-pressure steam sterilization 6.3 Preparation of glassware 6.4 Maintenance of stock cultures 7 Procedure 7.1 Biofilm formation on the surface of the test specimens 7.1.1 Pre-culture of bacteria 7.1.2 Preparation of test specimens
13	7.1.3 Preparation of the inoculated incubating medium 7.1.4 Inoculation of test specimens 7.1.5 Incubation of the inoculated test specimens
14	7.2 Determination of the amount of biofilm 7.2.1 First washing of test specimens 7.2.2 Staining of test specimens with crystal violet 7.2.3 Second washing of test specimens 7.2.4 Biofilm recovery from test specimens
15	7.2.5 Absorbance measurement 7.3 Determining the viable bacteria count 8 Expression of results 8.1 Conditions for valid test
16	8.2 Calculation of anti-biofilm activity on the basis of absorption spectrum method for anti-biofilm materials 8.3 Effectiveness of the anti-biofilm agent 9 Repeatability and reproducibility 10 Test report
17	Annex A (informative) Repeatability and reproducibility
19	Bibliography

Additional information

Status	Definitive
Pages	20
Publication Date	2023-07-27
ISBN	978 0 539 15206 7
Standard Number	BS ISO 4768:2023
Title	Measurement method of anti-biofilm activity on plastic and other non-porous surface
Identical National Standard Of	ISO 4768
Descriptors	Measurement, Plastics, Porous materials, Coated materials, Ceramics, Stainless steels, Rubber, Textiles, Stain tests, Plastic films
Publisher	BSI
Committee	PRI/21
ICS Codes	83.080.01 - Plastics in general

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BS ISO 4768:2023

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