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BSI 22/30362586 DC 2022

$13.70

BS EN ISO 15213-2. Microbiology of the food chain. Horizontal method for the detection and enumeration of Clostridium spp. – Part 2. Enumeration of Clostridium perfringens by colony-count technique

Published By Publication Date Number of Pages
BSI 2022 50
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PDF Catalog

PDF Pages PDF Title
1 30362586-NC.pdf
3 ISO_DIS 15213-2 ed.1 – id.71498 Enquiry PDF (en).pdf
6 Foreword
7 Introduction
9 1 Scope
10 2 Normative references
3 Terms and definitions
4 Principle
4.1 General
11 4.2 Preparation of dilutions
4.3 Enumeration
4.4 Confirmation
5 Culture media and reagents
6 Equipment and consumables
12 7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
13 9.2 Test portion, initial suspension and dilutions
9.3 Heat pre-treatment to select spores
9.4 Inoculation and incubation
14 9.5 Enumeration of typical colonies
9.6 Confirmation of C.€perfringens
9.6.1 Selection of colonies for confirmation
9.6.2 Acid phosphatase test
15 9.6.3 SIM agar test
9.6.4 Differentiation between human pathogenic and non-pathogenic C.€perfringens strains (optional)
9.6.5 Interpretation
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
16 11.2 Repeatability limit
11.3 Reproducibility limit
17 12 Test report
13 13 Quality assurance
18 Annex€A (normative) Flow diagram of the procedure
19 Annex€B (normative) Culture media and reagents
24 Annex€C (informative) Method validation studies and performance characteristics
28 Annex€D (informative) Molecular differentiation between pathogenic and non-pathogenic C.€perfringens
49 Bibliography
BSI 22/30362586 DC 2022
$13.70