BSI 22/30362586 DC 2022
$13.70
BS EN ISO 15213-2. Microbiology of the food chain. Horizontal method for the detection and enumeration of Clostridium spp. – Part 2. Enumeration of Clostridium perfringens by colony-count technique
Published By | Publication Date | Number of Pages |
BSI | 2022 | 50 |
PDF Catalog
PDF Pages | PDF Title |
---|---|
1 | 30362586-NC.pdf |
3 | ISO_DIS 15213-2 ed.1 – id.71498 Enquiry PDF (en).pdf |
6 | Foreword |
7 | Introduction |
9 | 1 Scope |
10 | 2 Normative references 3 Terms and definitions 4 Principle 4.1 General |
11 | 4.2 Preparation of dilutions 4.3 Enumeration 4.4 Confirmation 5 Culture media and reagents 6 Equipment and consumables |
12 | 7 Sampling 8 Preparation of test sample 9 Procedure 9.1 General |
13 | 9.2 Test portion, initial suspension and dilutions 9.3 Heat pre-treatment to select spores 9.4 Inoculation and incubation |
14 | 9.5 Enumeration of typical colonies 9.6 Confirmation of C.€perfringens 9.6.1 Selection of colonies for confirmation 9.6.2 Acid phosphatase test |
15 | 9.6.3 SIM agar test 9.6.4 Differentiation between human pathogenic and non-pathogenic C.€perfringens strains (optional) 9.6.5 Interpretation 10 Expression of results 11 Performance characteristics of the method 11.1 Interlaboratory study |
16 | 11.2 Repeatability limit 11.3 Reproducibility limit |
17 | 12 Test report 13 13 Quality assurance |
18 | Annex€A (normative) Flow diagram of the procedure |
19 | Annex€B (normative) Culture media and reagents |
24 | Annex€C (informative) Method validation studies and performance characteristics |
28 | Annex€D (informative) Molecular differentiation between pathogenic and non-pathogenic C.€perfringens |
49 | Bibliography |