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BSI 22/30453547 DC 2022

BSI 22/30453547 DC 2022

$13.70

BS EN ISO 20631. Infant formula and adult nutritionals. Determination of total folates content by trienzyme extraction and ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS)

Published By Publication Date Number of Pages
BSI 2022 25
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PDF Catalog

PDF Pages PDF Title
1 30453547-NC.pdf
3 ISO_DIS 20631 ed.1 – id.80495 Enquiry PDF (en) (1).pdf
6 Foreword
7 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
8 5 Reagents and materials
15 6 Apparatus
16 7 Procedure
7.1 Sample preparation
7.1.1 Sample processing to make them homogenous
7.1.2 Reconstitution of powder sample into liquid
17 7.2 Extraction
7.3 Extract purification
18 7.4 Instrumental analysis
7.4.1 Analysis of instrument blank, calibration standards, method blank and samples
7.4.2 LC conditions and MS parameters for different systems
19 8 Calculations
8.1 Calculation of ratios of peak areas of the folate compounds to the respective internal standard peak areas
8.2 Calibration curve
8.3 Calculation of concentration of folate compounds in the method blank
8.4 Calculation of concentration of folate compounds in the sample extracts
20 8.5 Calculation of folate compounds and total folate in reconstituted or RTF samples (μg/100 g)
21 8.6 Calculation of total folate in dry samples (μg/100 g) as-is basis
8.7 Calculation of folic acid nanograms released in the conjugase test
8.8 Calculation of percent conversion of Pte-Glu3 to folic acid in the conjugase assay
9 Precision
9.1 General
9.2 Repeatability
22 9.3 Reproducibility
23 Annex€A (informative) Example of a chromatogram of folate analysis
24 Annex€B (informative) Precision data
25 Bibliography

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BSI 22/30453547 DC 2022
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