🔥🔥 Don’t Miss Out on Our End of Autumn Sale. If you have any questions, feel free to click the bottom right corner to contact our customer service..

Concat us[email protected]
Shopping Cart

No products in the cart.

🔍
BSI PD ISO/TS 22690:2021

BSI PD ISO/TS 22690:2021

$102.76

Genomics informatics. Reliability assessment criteria for high-throughput gene-expression data

Published By Publication Date Number of Pages
BSI 2021 20
PDF Format Searchable Printable Preview Now
Guaranteed Safe Checkout
Categories: ,

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. We’re here to assist you 24/7.
Email:[email protected]

This document specifies reliability assessment criteria for high-throughput gene-expression data.

It is applicable to assessing the accuracy, reproducibility, and comparability of gene-expression data that are generated from microarray, next-generation sequencing, and other forms of high-throughput technologies.

This document identifies the quality-related data for the process of the next-generation sequencing of RNA (RNA-seq). The sequencing platform covered by this document is limited to short-read sequencers. The use of RNA-seq for mutation detection and virus identification is outside of the scope of this document.

This document is applicable to human health associated species such as human, cell lines, and preclinical animals. Other biological species are outside the scope of this document.

From a biological point of view, expression profiles of all genetic sequences including genes, transcripts, isoforms, exons, and junctions are within the scope of this document

PDF Catalog

PDF Pages PDF Title
2 undefined
5 Foreword
6 Introduction
8 1 Scope
2 Normative references
3 Terms and definitions
10 4 From sample to RNA
4.1 General
4.2 RNA integrity
11 4.3 RNA concentration
4.4 RNA purity
5 Expression profiling
6 Quality control metrics in RNA-seq analysis
6.1 General
6.2 Sequencing read
6.2.1 Total number of reads
12 6.2.2 Read length
6.2.3 Base call quality
6.2.4 GC content
6.2.5 Overrepresented sequence
6.2.6 Adapter residue
6.3 Alignment
6.3.1 Alignment ratio
6.3.2 Gene body coverage uniformity
13 6.3.3 Strand specificity
6.3.4 Insert size
6.3.5 Mismatch
6.3.6 Contamination from other sources
6.4 Expression
6.4.1 Expression distribution
6.4.2 Expressed genes
14 6.4.3 Saturation
6.4.4 Reproducibility
6.5 Differentially expressed genes
6.6 Biological interpretation of differentially expressed genes
15 6.7 Sample certificate of origin
6.8 Quality control of batch effects
7 Spike-in controls
8 Proficiency testing
8.1 General
16 8.2 RNA sources
8.3 Experimental design
9 Process management
17 Bibliography

Related products

BSI PD ISO/TS 22690:2021
$102.76