DIN EN ISO 15216-2:2019 Edition
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Mikrobiologie der Lebensmittelkette – Horizontales Verfahren zur Bestimmung von Hepatitis-A-Virus und Norovirus in Lebensmitteln mittels Real-time-RT-PCR – Teil 2: Nachweisverfahren
Published By | Publication Date | Number of Pages |
DIN | 2019-12 | 58 |
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In diesem Dokument wird ein Verfahren zum Nachweis von HAV und Noroviren der Genogruppen I (GI) und II (GII) in Untersuchungsproben von Lebensmitteln oder Lebensmitteloberflächen beschrieben. Nach der Freisetzung der Viren aus der Untersuchungsprobe wird die virale RNA durch Aufschluss des Viruskapsids mit Guanidinthiocyanat und Adsorption an Silica(-Partikeln) extrahiert. Die Zielsequenzen innerhalb der viralen RNA werden amplifiziert und mittels Real-time RT PCR nachgewiesen.*Inhaltsverzeichnis
Published Code | DIN |
---|---|
Published By | Deutsches Institut für Normung |
Publication Date | 2019-12 |
Pages Count | 58 |
Language | Germany |
Descriptors | Bakterienviren, Begriffe, Definition, Echtzeit, Futtermittel, Hepatitis, Kontamination, Laborprüfung, Laboruntersuchung, Lebensmittel, Lebensmittelprüfung, Lebensmitteluntersuchung, mikrobielle Lebensmittel, mikrobiologische Analyse, Mikroorganismen, Nachweis, Norovirus, Oberfläche, PCR, Polymerase, qualitative Analyse, Untersuchung, Virus |
File Size | 2.4 MB |
ICS Codes | 07.100.30 - Food microbiology |
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