ISO/TS 20224-2:2020

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Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 2: Ovine DNA detection method

Published By	Publication Date	Number of Pages
ISO	2020-07	24

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Categories: 67.050 - General methods of tests and analysis for food products, ISO

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Description

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of ovine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of ovine material derived from sheep (Ovis aries).

The target sequence is a partial fragment of the ovine nuclear prolactin receptor short form mRNA gene (PRLR) (i.e. GenBank accession number AF041979.1)^[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with = 95 % replicability at this concentration (LOD_{95 %}).

Additional information

Published Code	ISO
Published By	International Organization for Standardization
Publication Date	2020-07
Pages Count	24
Language	English
Edition	1
File Size	829.4 KB
ICS Codes	67.050 - General methods of tests and analysis for food products

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ISO/TS 20224-2:2020

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