{"id":341109,"date":"2024-10-19T23:54:42","date_gmt":"2024-10-19T23:54:42","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-iso-15216-12017\/"},"modified":"2024-10-25T23:05:53","modified_gmt":"2024-10-25T23:05:53","slug":"bs-en-iso-15216-12017","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-iso-15216-12017\/","title":{"rendered":"BS EN ISO 15216-1:2017"},"content":{"rendered":"

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
7<\/td>\nForeword <\/td>\n<\/tr>\n
8<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
11<\/td>\n4 Principle
4.1 Virus extraction <\/td>\n<\/tr>\n
12<\/td>\n4.2 RNA extraction
4.3 Real-time RT-PCR
4.4 Control materials
4.4.1 Process control virus
4.4.2 Double-stranded DNA (dsDNA) control
4.4.3 EC RNA control <\/td>\n<\/tr>\n
13<\/td>\n4.5 Test results
5 Reagents
5.1 General
5.2 Reagents used as supplied <\/td>\n<\/tr>\n
14<\/td>\n5.3 Prepared reagents <\/td>\n<\/tr>\n
15<\/td>\n6 Equipment and consumables <\/td>\n<\/tr>\n
17<\/td>\n7 Sampling
8 Procedure
8.1 General laboratory requirements
8.2 Virus extraction
8.2.1 Process control virus material
8.2.2 Negative process control
8.2.3 Food surfaces
8.2.4 Soft fruit, leaf, stem and bulb vegetables <\/td>\n<\/tr>\n
18<\/td>\n8.2.5 Bottled water <\/td>\n<\/tr>\n
19<\/td>\n8.2.6 Bivalve molluscan shellfish
8.3 RNA extraction <\/td>\n<\/tr>\n
20<\/td>\n8.4 Real-time RT-PCR
8.4.1 General requirements
8.4.2 Real-time RT-PCR analysis <\/td>\n<\/tr>\n
22<\/td>\n9 Interpretation of results
9.1 General
9.2 Construction of standard curves <\/td>\n<\/tr>\n
23<\/td>\n9.3 Calculation of RT-PCR inhibition
9.4 Calculation of extraction efficiency <\/td>\n<\/tr>\n
24<\/td>\n9.5 Sample quantification <\/td>\n<\/tr>\n
25<\/td>\n10 Expression of results
11 Precision
11.1 Interlaboratory study
11.2 Repeatability <\/td>\n<\/tr>\n
26<\/td>\n11.3 Reproducibility limit
12 Test report <\/td>\n<\/tr>\n
27<\/td>\nAnnex\u00a0A (normative) Diagram of procedure <\/td>\n<\/tr>\n
28<\/td>\nAnnex\u00a0B (normative) Composition and preparation of reagents and buffers <\/td>\n<\/tr>\n
31<\/td>\nAnnex\u00a0C (informative) Real-time RT-PCR mastermixes and cycling parameters <\/td>\n<\/tr>\n
32<\/td>\nAnnex\u00a0D (informative) Real-time RT-PCR primers and hydrolysis probes for the detection of HAV, norovirus GI and GII and mengo virus (process control) <\/td>\n<\/tr>\n
35<\/td>\nAnnex\u00a0E (informative) Growth of mengo virus strain MC0 for use as a process control <\/td>\n<\/tr>\n
36<\/td>\nAnnex\u00a0F (informative) RNA extraction using the NucliSENS\u00ae system <\/td>\n<\/tr>\n
38<\/td>\nAnnex\u00a0G (informative) Generation of dsDNA control stocks <\/td>\n<\/tr>\n
41<\/td>\nAnnex\u00a0H (informative) Generation of EC RNA stocks <\/td>\n<\/tr>\n
43<\/td>\nAnnex\u00a0I (informative) Typical optical plate layout <\/td>\n<\/tr>\n
45<\/td>\nAnnex\u00a0J (informative) Method validation studies and performance characteristics <\/td>\n<\/tr>\n
56<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Microbiology of the food chain. Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR – Method for quantification<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2017<\/td>\n60<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":341117,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-341109","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/341109","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/341117"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=341109"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=341109"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=341109"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}