{"id":641792,"date":"2024-11-06T01:25:17","date_gmt":"2024-11-06T01:25:17","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/iso-ts-131362012\/"},"modified":"2024-11-06T01:25:17","modified_gmt":"2024-11-06T01:25:17","slug":"iso-ts-131362012","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/iso\/iso-ts-131362012\/","title":{"rendered":"ISO\/TS 13136:2012"},"content":{"rendered":"<p>ISO\/TR 13136:2012 describes the identification of Shiga toxin-producing <i>Escherichia coli <\/i>(STEC) by means of the detection of the following genes: a) the major virulence genes of STEC, <i>stx<\/i> and <i>eae<\/i>; b) the genes associated with the serogroups O157, O111, O26, O103, and O145.<\/p>\n<p>In any case, when one or both of the <i>stx<\/i> genes is\/are detected, the isolation of the strain is attempted.<\/p>\n<p>The isolation of STEC from samples positive for the presence of the genes specifying the serogroups in the scope of this method can be facilitated by using serogroup-specific enrichment techniques (e.g. immunomagnetic separation, IMS).<\/p>\n<p>The protocol uses real-time PCR as the reference technology for detection of the virulence and serogroup-associated genes.<\/p>\n<p>ISO\/TR 13136:2012 is applicable to: 1) products intended for human consumption and the feeding of animals; 2) environmental samples in the area of food production and food handling; 3) environmental samples in the area of primary production.<\/p>\n","protected":false},"excerpt":{"rendered":"<p><b>Microbiology of food and animal feed \u2014 Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens \u2014 Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups<\/b><\/p>\n<table style=\"width: 100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Published By<\/td>\n<td>Publication Date<\/td>\n<td>Number of Pages<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/pdfstandards.shop\/product-category\/publishers\/iso\/\"><b>ISO<\/b><\/a><\/td>\n<td>2012-11<\/td>\n<td>30<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":641806,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2634],"product_tag":[],"class_list":{"0":"post-641792","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-iso","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/641792","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/641806"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=641792"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=641792"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=641792"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}