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BS EN 13098:2019

BS EN 13098:2019

$189.07

Workplace exposure. Measurement of airborne microorganisms and microbial compounds. General requirements

Published By Publication Date Number of Pages
BSI 2019 46
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This document specifies general requirements for the measurement of microorganisms and microbial compounds. This document provides also guidelines for the assessment of workplace exposure to airborne microorganisms including the determination of total number and culturable number of microorganisms and microbial compounds in the workplace atmosphere. This document does not apply to the measurement of viruses.

PDF Catalog

PDF Pages PDF Title
2 undefined
8 1 Scope
2 Normative references
3 Terms and definitions
11 4 Symbols and abbreviations
5 Measurement of microorganisms and microbial compounds
5.1 Biological agents and biological properties
12 5.2 Aim of measurement
5.3 Measurement strategy
5.3.1 General
5.3.2 Specification of the objectives for measurement
5.3.3 Specification of the measurement task
13 5.3.4 Collection of background information
5.3.5 Sampling strategy
5.4 Measurement options
14 5.5 Uncertainty of measurement
5.6 Variability of exposure level
6 Sampling
6.1 Principles and general requirements
15 6.2 Sampler
6.2.1 Categories
6.2.2 Requirements
6.3 Pumps
6.4 Operator skills
6.5 Transport and storage of samples
6.5.1 General
16 6.5.2 Transport
6.5.3 Storage at the laboratory
6.6 Sampling documentation
17 7 Analytical method
7.1 Requirements
7.2 Validation
7.3 Documentation
7.3.1 General information
18 7.3.2 Specific information
7.3.2.1 General
7.3.2.2 Analysis of culturable microorganisms
7.3.2.3 Analysis by microscopy
7.3.2.4 Analysis of microbial compounds and metabolites
19 7.4 Determination of culturable fraction
7.5 Determination of direct cell count by microscopy
7.5.1 General
7.5.2 Epifluorescence microscopy and light microscopy
7.5.3 Scanning electron microscopy
7.6 Determination of microbial compounds
8 Expression of results
8.1 General
8.2 Cultivation methods
20 8.3 Microscopic methods
8.4 Microbial compounds
9 Test report
21 Annex A (informative)Recommendations for selection of exposure measuring procedures
33 Annex B (informative)Sampling form example
35 Annex C (normative)Determination of airborne microorganisms by cultivation
C.1 General
C.2 Requirements
C.2.1 Suspension media and dilution media
C.2.2 Cultivation media
C.2.3 Cultivation temperature and incubation period
36 C.2.4 Colony counts
C.2.5 Identification
37 Annex D (informative)List of generic media
38 Annex E (informative)Formula and calculation examples for colony counting
E.1 Calculation
39 E.2 Examples

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BS EN 13098:2019
$189.07