BS ISO 13019:2018
$142.49
Tissue-engineered medical products. Quantification of sulfated glycosaminoglycans (sGAG) for evaluation of chondrogenesis
| Published By | Publication Date | Number of Pages |
| BSI | 2018 | 24 |
This document specifies test methods for the quantification of sulfated glycosaminoglycans (sGAG), one of the major extracellular matrix components in articular, meniscal, and elastic cartilages, and tissue-engineered cartilage constructs.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | National foreword |
| 6 | Foreword |
| 7 | 1 Scope 2 Normative references 3 Terms and definitions |
| 8 | 4 General and principle 5 Apparatus |
| 9 | 6 Preparation of parallel test samples from test samples 6.1 General 6.2 Preparation of parallel test samples 7 Procedure 7.1 General 7.2 Procedure for pre-treatment of parallel test samples 7.2.1 Procedure for dissection |
| 10 | 7.3 Procedure for extraction of sGAG from pre-treated parallel test samples 7.3.1 General 7.3.2 Procedure for papain digestion 7.4 Procedure for measurement of sGAG content of parallel test samples by DMMB assay 7.4.1 Principle 7.4.2 Procedure for preparation of 1,9-dimethylmethylene blue (DMMB) solution |
| 11 | 7.4.3 Procedure for DMMB assay 7.5 Normalization of sGAG contents |
| 12 | 8 Test report |
| 13 | 9 Evaluation |
| 14 | Annex A (informative) Flowchart of the overall procedure steps |
| 15 | Annex B (informative) Reagents |
| 16 | Annex C (informative) Alternative procedure for pre-treatment |
| 17 | Annex D (informative) Alternative procedure for extraction of sGAG |
| 20 | Annex E (informative) Example of test report format for sGAG measurement |
| 22 | Bibliography |
