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BSI PD CEN ISO/TS 17919:2013

BSI PD CEN ISO/TS 17919:2013

$198.66

Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of botulinum type A, B, E and F neurotoxin-producing clostridia

Published By Publication Date Number of Pages
BSI 2013 58
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This Technical Specification specifies a horizontal method for the molecular detection of clostridia carrying botulinum neurotoxin A, B, E, and F genes by a PCR method. This method detects the genes and not the toxins, therefore a positive result does not necessarily mean the presence of these toxins in the sample investigated. This Technical Specification is applicable to products for human consumption, animal feed, and environmental samples.

The PCR assays for detection of genetic sequences encoding specific toxin types are described in Annexes B and C.

PDF Catalog

PDF Pages PDF Title
4 Foreword
6 Foreword
7 Introduction
9 Section sec_1
Section sec_2
Section sec_3
Section sec_4
Section sec_4.1
1 Scope
2 Normative references
3 Terms and definitions
4 Symbols and abbreviated terms
4.1 Symbols
10 Section sec_4.2
Section sec_5
Section sec_5.1
Section sec_5.2
Section sec_5.3
Section sec_5.4
Section sec_5.5
Section sec_5.6
4.2 Abbreviated terms
5 Principle
5.1 General
5.2 Microbial enrichment
5.3 Nucleic acid extraction
5.4 Amplification by PCR
5.5 Detection of PCR products
5.6 Confirmation
11 Section sec_6
Section sec_6.1
Section sec_6.2
Section sec_6.2.1
Section sec_6.2.2
Section sec_6.2.3
Section sec_6.2.3.1
Section sec_6.2.3.2
Section sec_6.2.3.3
Section sec_6.2.4
Section sec_6.2.4.1
Section sec_6.2.4.1.1
6 Reagents
6.1 General
6.2 Culture media
12 Section sec_6.2.4.1.2
Section sec_6.2.4.1.3
Section sec_6.2.4.2
Section sec_6.3
Section sec_6.3.1
Section sec_6.3.2
Section sec_6.3.3
Section sec_6.3.4
Section sec_6.3.5
Section sec_6.3.6
Section sec_6.3.7
Section sec_6.3.8
Section sec_6.3.9
Section sec_6.3.10
Section sec_6.3.11
Section sec_6.3.12
Section sec_6.3.13
6.3 Nucleic acid extraction
13 Section sec_6.3.14
Section sec_6.3.15
Section sec_6.3.16
Section sec_6.4
Section sec_6.4.1
Section sec_6.4.2
Section sec_6.4.3
Section sec_6.4.4
Section sec_7
Section sec_7.1
Section sec_7.2
Section sec_7.2.1
Section sec_7.2.2
Section sec_7.2.3
Section sec_7.2.4
Section sec_7.3
Section sec_7.3.1
Section sec_7.3.2
Section sec_7.3.3
6.4 Reagents for PCR
7 Apparatus and equipment
7.1 General
7.2 Equipment for sample preparation prior to enrichment
7.3 Equipment for microbial enrichment
14 Section sec_7.3.4
Section sec_7.4
Section sec_7.4.1
Section sec_7.4.2
Section sec_7.4.3
Section sec_7.4.4
Section sec_7.4.5
Section sec_7.5
Section sec_7.5.1
Section sec_7.5.2
Section sec_7.5.3
Section sec_7.5.4
Section sec_7.6
Section sec_7.6.1
Section sec_7.6.1.1
Section sec_7.6.1.2
Section sec_7.6.1.3
Section sec_7.6.1.4
Section sec_7.6.2
Section sec_7.6.2.1
Section sec_7.6.2.2
7.4 Equipment used for nucleic acid extraction
7.5 Equipment used for PCR
7.6 Equipment used for the detection of the PCR product
15 Section sec_8
Section sec_9
Section sec_9.1
Section sec_9.1.1
Section sec_9.1.2
Section sec_9.1.3
Section sec_9.2
Section sec_9.2.1
Section sec_9.2.1.1
Section sec_9.2.1.2
Section sec_9.2.1.2.1
8 Sampling
9 Procedure
9.1 Sample preparation prior to enrichment
9.2 Microbial enrichment
16 Section sec_9.2.1.2.2
Section sec_9.2.1.3
Section sec_9.2.2
Section sec_9.2.3
Section sec_9.3
Section sec_9.3.1
Section sec_9.3.2
9.3 Nucleic acid preparation
17 Section sec_9.3.3
Section sec_9.3.4
Section sec_9.4
Section sec_9.4.1
Section sec_9.4.2
Section sec_9.5
Section sec_9.5.1
9.4 PCR amplification
9.5 Confirmation of a positive PCR result
18 Annex sec_A
Figure fig_A.1
Annex A
(normative)

Flow-diagram of the procedure

19 Annex sec_B
Annex sec_B.1
Annex sec_B.1.1
Annex sec_B.1.2
Annex sec_B.1.2.1
Annex sec_B.1.2.2
Annex sec_B.1.2.3
Annex sec_B.1.2.3.1
Annex B
(informative)

Multiplex PCR assays for detection of genes encoding botulinum neurotoxin types A, B, E, and F using agarose gel electrophoresis

20 Table tab_B.1
Annex sec_B.1.2.3.2
21 Table tab_B.2
Annex sec_B.1.2.4
Annex sec_B.1.2.4.1
Annex sec_B.1.2.4.2
Annex sec_B.1.2.5
22 Annex sec_B.1.2.6
Annex sec_B.1.3
Annex sec_B.1.4
Annex sec_B.1.4.1
Annex sec_B.1.4.2
Annex sec_B.1.4.2.1
Annex sec_B.1.4.2.2
Annex sec_B.1.4.2.3
Annex sec_B.1.4.2.4
Annex sec_B.1.4.2.5
Annex sec_B.1.4.2.6
23 Table tab_B.3
Annex sec_B.1.4.3
Annex sec_B.1.4.3.1
Annex sec_B.1.4.3.2
Annex sec_B.1.4.3.3
Annex sec_B.1.4.3.4
Annex sec_B.1.4.3.5
Annex sec_B.1.4.3.6
Annex sec_B.1.4.3.7
Annex sec_B.1.4.3.8
Annex sec_B.1.4.3.9
Annex sec_B.1.4.3.10
Annex sec_B.1.4.3.11
Annex sec_B.1.4.3.12
Annex sec_B.1.4.3.13
Annex sec_B.1.4.3.14
24 Annex sec_B.1.4.3.15
Annex sec_B.1.4.3.16
Annex sec_B.1.4.3.17
Annex sec_B.1.5
Annex sec_B.1.5.1
Annex sec_B.1.5.2
Annex sec_B.1.5.2.1
Annex sec_B.1.5.2.2
Annex sec_B.1.5.2.3
Annex sec_B.1.5.2.4
Annex sec_B.1.5.2.5
Annex sec_B.1.5.2.6
Annex sec_B.1.5.2.7
Annex sec_B.1.5.2.8
Annex sec_B.1.5.2.9
Annex sec_B.1.5.2.10
25 Annex sec_B.1.6
Annex sec_B.1.6.1
Annex sec_B.1.6.2
Annex sec_B.1.6.2.1
Annex sec_B.1.6.2.2
Annex sec_B.1.6.2.3
Annex sec_B.1.6.2.4
Annex sec_B.1.6.2.5
Annex sec_B.1.6.2.6
Annex sec_B.1.6.2.7
Annex sec_B.1.6.2.8
Annex sec_B.1.6.2.9
Table tab_B.4
Annex sec_B.1.6.3
26 Table tab_B.5
Annex sec_B.1.6.4
Table tab_B.6
Annex sec_B.1.6.5
Annex sec_B.1.7
Annex sec_B.1.7.1
27 Table tab_B.7
Annex sec_B.1.7.2
Annex sec_B.1.7.2.1
Annex sec_B.1.7.2.2
Annex sec_B.1.7.2.3
Annex sec_B.1.7.2.4
Annex sec_B.1.7.3
28 Table tab_B.8
Annex sec_B.1.7.4
Annex sec_B.1.7.4.1
Annex sec_B.1.7.4.2
Annex sec_B.1.7.4.3
Annex sec_B.1.7.4.4
29 Annex sec_B.1.7.4.5
Table tab_B.9
Annex sec_B.1.7.5
Annex sec_B.1.7.6
Annex sec_B.2
Annex sec_B.2.1
Annex sec_B.2.2
Annex sec_B.2.2.1
30 Annex sec_B.2.2.2
Annex sec_B.2.2.3
Annex sec_B.2.2.3.1
Table tab_B.10
Annex sec_B.2.2.3.2
31 Table tab_B.11
Annex sec_B.2.2.4
Annex sec_B.2.2.5
Annex sec_B.2.2.6
32 Annex sec_B.2.3
Annex sec_B.2.4
Annex sec_B.2.4.1
Annex sec_B.2.4.2
Annex sec_B.2.4.2.1
Annex sec_B.2.4.2.2
Annex sec_B.2.4.2.3
Annex sec_B.2.4.2.4
Annex sec_B.2.4.2.5
Annex sec_B.2.4.2.6
Table tab_B.12
Annex sec_B.2.4.3
Annex sec_B.2.5
33 Annex sec_B.2.6
Annex sec_B.2.6.1
Annex sec_B.2.6.2
Annex sec_B.2.6.2.1
Annex sec_B.2.6.2.2
Annex sec_B.2.6.2.3
Annex sec_B.2.6.2.4
Annex sec_B.2.6.2.5
Annex sec_B.2.6.2.6
Annex sec_B.2.6.2.7
Annex sec_B.2.6.2.8
Annex sec_B.2.6.2.9
Table tab_B.13
Annex sec_B.2.6.3
34 Table tab_B.14
Annex sec_B.2.6.4
Table tab_B.15
Annex sec_B.2.6.5
Annex sec_B.2.7
Annex sec_B.2.7.1
35 Table tab_B.16
Annex sec_B.2.7.2
Annex sec_B.2.7.2.1
Annex sec_B.2.7.2.2
Annex sec_B.2.7.2.3
Annex sec_B.2.7.2.4
Annex sec_B.2.7.3
Table tab_B.17
36 Annex sec_B.2.7.4
Annex sec_B.2.7.4.1
Annex sec_B.2.7.4.2
Table tab_B.18
Annex sec_B.2.7.5
Annex sec_B.2.7.6
37 Annex sec_C
Annex sec_C.1
Annex sec_C.1.1
Annex sec_C.1.2
Annex sec_C.1.2.1
Annex sec_C.1.2.2
Annex sec_C.1.2.3
Annex sec_C.1.2.3.1
Annex C
(informative)

Assays for detection of genes encoding botulinum neurotoxin types A, B, E, and F using real-time PCR

38 Table tab_C.1
Annex sec_C.1.2.3.2
Table tab_C.2
Annex sec_C.1.2.4
Annex sec_C.1.2.4.1
39 Annex sec_C.1.2.4.2
Annex sec_C.1.2.5
Annex sec_C.1.2.6
Annex sec_C.1.3
Annex sec_C.1.4
Annex sec_C.1.4.1
Annex sec_C.1.4.2
Annex sec_C.1.4.3
Annex sec_C.1.4.4
40 Annex sec_C.1.4.5
Table tab_C.3
Annex sec_C.1.5
Annex sec_C.1.5.1
Annex sec_C.1.5.2
Annex sec_C.1.5.2.1
Annex sec_C.1.5.2.2
Annex sec_C.1.5.2.3
Annex sec_C.1.5.2.4
41 Annex sec_C.1.6
Annex sec_C.1.6.1
Table tab_C.4
Table tab_C.5
Annex sec_C.1.6.2
Annex sec_C.1.6.2.1
Annex sec_C.1.6.2.2
42 Annex sec_C.1.6.2.3
Annex sec_C.1.6.2.4
Annex sec_C.1.6.3
Table tab_C.6
Annex sec_C.1.6.4
Annex sec_C.1.6.5
Annex sec_C.2
Annex sec_C.2.1
Annex sec_C.2.2
Annex sec_C.2.2.1
43 Annex sec_C.2.2.2
Annex sec_C.2.2.3
Annex sec_C.2.2.3.1
Table tab_C.7
Annex sec_C.2.2.3.2
Table tab_C.8
Annex sec_C.2.2.4
Annex sec_C.2.2.4.1
Annex sec_C.2.2.4.2
44 Annex sec_C.2.2.4.3
Annex sec_C.2.2.5
Annex sec_C.2.2.6
Annex sec_C.2.3
Annex sec_C.2.4
Annex sec_C.2.4.1
Annex sec_C.2.4.2
Annex sec_C.2.4.3
Annex sec_C.2.4.4
45 Table tab_C.9
Annex sec_C.2.4.5
Annex sec_C.2.4.5.1
Annex sec_C.2.4.5.2
Annex sec_C.2.4.5.3
Annex sec_C.2.5
Annex sec_C.2.5.1
46 Annex sec_C.2.5.2
Annex sec_C.2.5.2.1
Annex sec_C.2.5.2.2
Annex sec_C.2.5.2.3
Annex sec_C.2.5.2.4
Annex sec_C.2.6
Annex sec_C.2.6.1
Table tab_C.10
Table tab_C.11
47 Annex sec_C.2.6.2
Annex sec_C.2.6.2.1
Annex sec_C.2.6.2.2
Annex sec_C.2.6.2.3
Annex sec_C.2.6.2.4
Annex sec_C.2.6.3
Table tab_C.12
Annex sec_C.2.6.4
Annex sec_C.2.6.5
48 Annex sec_D
Annex sec_D.1
Annex sec_D.2
Annex sec_D.2.1
Annex sec_D.2.2
Annex sec_D.2.2.1
Annex sec_D.2.2.1.1
Annex sec_D.2.2.1.2
Annex sec_D.2.2.2
Annex sec_D.2.2.2.1
Annex D
(informative)

Preparation of C. botulinum spores

49 Annex sec_D.2.2.2.2
Annex sec_D.2.2.3
Annex sec_D.2.3
Annex sec_D.2.3.1
Annex sec_D.2.3.1.1
Annex sec_D.2.3.1.2
Annex sec_D.2.3.2
Annex sec_D.2.3.3
50 Annex sec_D.3
Annex sec_D.3.1
Annex sec_D.3.1.1
Annex sec_D.3.1.2
Annex sec_D.3.2
Annex sec_D.3.2.1
Annex sec_D.3.2.2
Annex sec_D.3.2.3
Annex sec_D.3.2.4
Annex sec_D.3.2.5
Annex sec_D.3.2.6
Annex sec_D.3.2.7
Annex sec_D.3.2.8
Annex sec_D.3.2.9
Annex sec_D.3.2.10
Annex sec_D.3.2.11
Annex sec_D.4
Annex sec_D.4.1
Annex sec_D.4.2
Annex sec_D.4.2.1
Annex sec_D.4.2.2
Annex sec_D.4.2.3
51 Annex sec_D.4.2.4
Annex sec_D.4.3
Annex sec_D.4.3.1
Annex sec_D.4.3.2
Annex sec_D.4.3.3
Annex sec_D.4.3.4
Annex sec_D.5
Annex sec_D.5.1
Annex sec_D.5.1.1
Annex sec_D.5.1.2
Annex sec_D.5.2
Annex sec_D.5.3
52 Annex sec_D.5.4
Annex sec_D.5.4.1
Annex sec_D.5.4.2
Annex sec_D.5.4.2.1
Annex sec_D.5.4.2.2
Annex sec_D.5.4.2.3
53 Figure fig_D.1
54 Reference ref_1
Reference ref_2
Reference ref_3
Reference ref_4
Reference ref_5
Reference ref_6
Reference ref_7
Reference ref_8
Reference ref_9
Reference ref_10
Reference ref_11
Reference ref_12
Reference ref_13
Bibliography
Bibliography
55 Reference ref_14
Reference ref_15
Reference ref_16
Reference ref_17

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