{"id":340472,"date":"2024-10-19T23:51:15","date_gmt":"2024-10-19T23:51:15","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-iso-221192011\/"},"modified":"2024-10-25T23:01:04","modified_gmt":"2024-10-25T23:01:04","slug":"bs-en-iso-221192011","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-iso-221192011\/","title":{"rendered":"BS EN ISO 22119:2011"},"content":{"rendered":"

This International Standard defines terms for the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). This International Standard also specifies requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real-time PCR.<\/p>\n

The minimum requirements laid down in this International Standard provide the basis for comparable and reproducible results within individual and between different laboratories.<\/p>\n

This International Standard is also applicable, for example, to the detection of food-borne pathogens in environmental samples and in animal feeding stuffs.<\/p>\n

\n

NOTE Because of the rapid progress in this field, the examples given are those most frequently in use at the time of development of this International Standard.<\/p>\n<\/blockquote>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
9<\/td>\nScope
Normative references
Terms and definitions <\/td>\n<\/tr>\n
13<\/td>\nPrinciple
General
Probes for real-time PCR
Hydrolysis probes
Hybridization probes <\/td>\n<\/tr>\n
14<\/td>\nMolecular beacons
General laboratory requirements
Reagents and materials <\/td>\n<\/tr>\n
15<\/td>\nApparatus <\/td>\n<\/tr>\n
16<\/td>\nLaboratory sample
Procedure
Sample preparation
Amplification
General
Cycling parameters
Hydrolysis probes
Hybridization probes <\/td>\n<\/tr>\n
17<\/td>\nMolecular beacons
Controls
Analysis of the fluorescence data
Amplification plot
General <\/td>\n<\/tr>\n
18<\/td>\nPhase 1: Exponential phase
Phase 2: Linear phase
Phase 3: Plateau phase
Evaluation of the fluorescence data
Quantitative analysis
General
Standard curve method for quantification <\/td>\n<\/tr>\n
19<\/td>\nEvaluation and documentation <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Microbiology of food and animal feeding stuffs. Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2011<\/td>\n24<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":340479,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-340472","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/340472","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/340479"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=340472"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=340472"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=340472"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}